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Rylan Larsen Lab: AAV GCaMP6 Plasmids

Unpublished

Plasmids from Article

ID Plasmid Purpose
105714pAAV-Ef1a-fDIO-GCaMP6sCan be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.
105715pAAV-Ef1a-DIO-Synaptophysin-GCaMP6sIn the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.
107736AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3In the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.
107737AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3In the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.
135418pAAV-TRE-GCaMP6sCan be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.
135419pAAV-Ef1a-Flex-Axon-GCaMP7bCan be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.

Antibodies from Article