Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity.

Moreb EA, Hoover B, Yaseen A, Valyasevi N, Roecker Z, Menacho-Melgar R, Lynch MD
ACS Synth Biol. 2017 Oct 2. doi: 10.1021/acssynbio.7b00174. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
89951	pKDsgRNA-malG	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.
89952	pKDsgRNA-arsB	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.
89953	pKDsgRNA-rpsL	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.
89954	pKDsgRNA-hisG	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.
89955	pKDsgRNA-trmI	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.
89956	pKDsgRNA-torZ	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.
89957	pKDsgRNA-rssA	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.
89958	pKDsgRNA-yhhT	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.
89959	pKDsgRNA-lpoB	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.
89960	pKDsgRNA-NT	Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.
89961	pEM-Cas9HF1	Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
89962	pEM-Cas9HF1-recA56	Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.
89963	pKDsgRNA-rbsK	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.
89964	pKDsgRNA-xylA	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.
89965	pKDsgRNA-srlA	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.
102283	pSMART-SOS-GFPuv	Fluorescent sensor of E. coli SOS response
102284	pKDsgRNA-pfkB	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.
102285	pEM-Cas9noSsrA	Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.
102286	pKDsgRNA-fsaA	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.
102288	pKDsgRNA-ldhA	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.
102290	pKDsgRNA-adhE	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.
102291	pKDsgRNA-ompT	Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.
102294	pEM-Cas9HF1-indRecA56	Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.