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Genome-wide CRISPR-KO Screen Uncovers mTORC1-Mediated Gsk3 Regulation in Naive Pluripotency Maintenance and Dissolution.

Li M, Yu JSL, Tilgner K, Ong SH, Koike-Yusa H, Yusa K
Cell Rep. 2018 Jul 10;24(2):489-502. doi: 10.1016/j.celrep.2018.06.027. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
105015pKLV2-U6gRNA5(Tcf7l1-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP
105016pKLV2-U6gRNA5(Tcf7l1-g2)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP
105017pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP
105018pKLV2-U6gRNA5(Pggt1b-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Pggt1b , co-expression of TagBFP
105019pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFP
105020pKLV2-U6gRNA5(Znrf1-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Znrf1 , co-expression of TagBFP
105021pKLV2-U6gRNA5(Zfp219-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Zfp219 , co-expression of TagBFP
105022pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP
105023pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP
105024pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP
105025pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP
105026pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP
105027pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP
105028pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP
105029pKLV2-U6gRNA5(Slc25a51-g2)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP
105030pKLV2-U6gRNA5(Ndufa2-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP
105031pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP
105032pKLV2-U6gRNA5(Hgs-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Hgs , co-expression of TagBFP
105033pKLV2-U6gRNA5(Vps25-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Vps25 , co-expression of TagBFP
105034pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP
105035pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP
105036pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP
105037pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP
105038pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP
105039pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP
105040pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP
105041pKLV2-U6gRNA5(Rictor-g1)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP
105042pKLV2-U6gRNA5(Rictor-g2)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP
105043pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-WLentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

Antibodies from Article