Membrane constriction and thinning by sequential ESCRT-III polymerization.
Nguyen HC, Talledge N, McCullough J, Sharma A, Moss FR 3rd, Iwasa JH, Vershinin MD, Sundquist WI, Frost A
Nat Struct Mol Biol. 2020 Apr;27(4):392-399. doi: 10.1038/s41594-020-0404-x. Epub 2020 Apr 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108284 | pGEX2T-TEV CHMP1B 4-178 | Expresses residues 4-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-279 |
| 108285 | pGEX2T-TEV CHMP1B 186-199 | Expresses residues 186-199 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-283 |
| 108286 | pGEX2T-TEV CHMP1B 1-143 | Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278 |
| 108287 | pGEX2T-TEV CHMP1B 144-178 | Expresses residues 144-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-281 |
| 108288 | pGEX2T-TEV CHMP1B 186-199 L188A/L192A | Expresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286 |
| 108289 | pGEX2T-TEV CHMP1B 4-199 L188A/L192A | Expresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284 |
| 108290 | pGEX2T-TEV CHMP1B 186-199 L192A/L195A | Expresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287 |
| 108291 | pGEX2T-TEV CHMP1B 4-199 L192A/L195A | Expresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285 |
| 115325 | pCA528 CHMP1B 4-199 | Expresses residues 4-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-23 |
| 115326 | pCA528 CHMP1B 1-199 | Expresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24 |