Expression of IgG Monoclonals with Engineered Immune Effector Functions.
Vazquez-Lombardi R, Nevoltris D, Rouet R, Christ D
Methods Mol Biol. 2018;1827:313-334. doi: 10.1007/978-1-4939-8648-4_16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104579 | pRVL-1 | Contains mouse kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme. |
| 104580 | pRVL-2 | Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions |
| 104581 | pRVL-3 | Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions |
| 104582 | pRVL-4 | Contains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme. |
| 104583 | pRVL-5 | Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions |
| 104584 | pRVL-6 | Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions |