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Identifying the functional contribution of the defatty-acylase activity of SIRT6.

Zhang X, Khan S, Jiang H, Antonyak MA, Chen X, Spiegelman NA, Shrimp JH, Cerione RA, Lin H
Nat Chem Biol. 2016 Aug;12(8):614-20. doi: 10.1038/nchembio.2106. Epub 2016 Jun 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
102322pCMV4a-SIRT6-FLAGHuman SIRT6 with FLAG tag for mammalian expression
102323pCMV4a-SIRT6-G60A-FLAGHuman SIRT6 G60A with FLAG tag for mammalian expression
102324pCMV4a-SIRT6-H133Y-FLAGHuman SIRT6 H133Y with FLAG tag for mammalian expression
102325pCDH-SIRT6Human SIRT6 for mammalian expression
102326pCDH-SIRT6-G60AHuman SIRT6 G60A for mammalian expression
102327pCDH-SIRT6-H133YHuman SIRT6 H133Y for mammalian expression

Antibodies from Article