TSPAN12 Is a Norrin Co-receptor that Amplifies Frizzled4 Ligand Selectivity and Signaling.
Lai MB, Zhang C, Shi J, Johnson V, Khandan L, McVey J, Klymkowsky MW, Chen Z, Junge HJ
Cell Rep. 2017 Jun 27;19(13):2809-2822. doi: 10.1016/j.celrep.2017.06.004.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115784 | pcDNA 3.3 HA-hTSPAN12 | Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites. |
| 115785 | pcDNA 3.3 HA-TSPAN12^11-LEL | Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence |
| 115786 | pcDNA 3.3 EGFP | Expresses EGFP in mammalian cells |
| 115787 | pcDNA 3.3 ss-V5-hFZD4 (aa 37-537) | Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag. |
| 115788 | pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615) | Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag. |
| 115789 | pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133) | Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags. |
| 115907 | pcDNA 3.3 LRP5 | Expresses human LRP5 in mammalian cells. |
| 115908 | pcDNA 3.3 hFZD4 | Expresses human FZD4 in mammalian cells. |