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An expanded tool kit for the auxin-inducible degron system in budding yeast.

Morawska M, Ulrich HD
Yeast. 2013 Sep;30(9):341-51. doi: 10.1002/yea.2967. Epub 2013 Jul 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
99513pHyg-AID(1-114)Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae
99514pHyg-AID(31-114)Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae
99515pHyg-AID*C-terminal AID* degron cassette with HygR marker for S. cerevisiae
99516pHyg-AID(1-114)-8mycTruncated C-terminal AID-8myc degron cassette with HygR marker for S. cerevisiae
99517pHyg-AID(31-114)-9mycTruncated C-terminal AID-9myc degron cassette with HygR marker for S. cerevisiae
99518pHyg-AID*-9mycC-terminal AID*-9myc degron cassette with HygR marker for S. cerevisiae
99519pHyg-AID*-6FLAGC-terminal AID*-6FLAG degron cassette with HygR marker for S. cerevisiae
99520pHyg-AID*-6HAC-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae
99521pHyg-AID*-GFPC-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiae
99522pKan-AID*-9mycC-terminal AID*-9myc degron cassette with KanR marker for S. cerevisiae
99523pNat-AID*-9mycC-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae
99524pHis-AID*-9mycC-terminal AID*-9myc degron cassette with HIS3MX marker for S. cerevisiae
99525pKan-PCUP1-AID*(N)N-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae
99526pKan-PCUP1-AID*(N)-9mycN-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiae
99527pKan-PCUP1-9myc-AID*(N)N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae
99528pKan-PRFA1-9myc-AID*(N)N-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiae

Antibodies from Article