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PurposeC-terminal AID*-6FLAG degron cassette with HygR marker for S. cerevisiae
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99519 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a derivative
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Vector typePCR template for yeast tagging cassette
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIAA17(71-116)
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Alt nameAID (Auxin-inducible degron)
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Alt nameAXR3
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SpeciesA. thaliana (mustard weed)
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Entrez GeneAXR3 (a.k.a. AT1G04250, AUXIN RESISTANT 3, AtIAA17, F19P19.31, F19P19_31, IAA17, indole-3-acetic acid inducible 17)
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Tag
/ Fusion Protein
- 6xFLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer CGTACGCTGCAGGTCGAC
- 3′ sequencing primer TCGATGAATTCGAGCTCG (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe parent vector encoding AID was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHyg-AID*-6FLAG was a gift from Helle Ulrich (Addgene plasmid # 99519 ; http://n2t.net/addgene:99519 ; RRID:Addgene_99519) -
For your References section:
An expanded tool kit for the auxin-inducible degron system in budding yeast. Morawska M, Ulrich HD. Yeast. 2013 Sep;30(9):341-51. doi: 10.1002/yea.2967. Epub 2013 Jul 23. 10.1002/yea.2967 PubMed 23836714