Engineering posttranslational proofreading to discriminate nonstandard amino acids.
Kunjapur AM, Stork DA, Kuru E, Vargas-Rodriguez O, Landon M, Soll D, Church GM
Proc Natl Acad Sci U S A. 2018 Jan 16;115(3):619-624. doi: 10.1073/pnas.1715137115. Epub 2018 Jan 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98564 | C321.Ub-UAG-sfGFP | Recoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter |
| 98565 | C321.ΔClpS.Ub-UAG-sfGFP | Recoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter and ClpS inactivated |
| 98566 | pZE21/UBP1/ClpS | Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon |
| 98567 | pZE21/UBP1/ClpS_V65I | Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon |