Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut.
Lim B, Zimmermann M, Barry NA, Goodman AL
Cell. 2017 Apr 20;169(3):547-558.e15. doi: 10.1016/j.cell.2017.03.045.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 90317 | pNBU2_erm_P1T_DP-GH023 | Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 |
| 90318 | pNBU2_erm_P1T_DP-A21 | Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS A21 |
| 90319 | pNBU2_erm_P1T_DP-B1 | Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B1 |
| 90320 | pNBU2_erm_P1T_DP-rpiL* | Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS rpiL* |
| 90321 | pNBU2_erm_P1T_DP-B41 | Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B41 |
| 90324 | pNBU2_erm-TetR-P1T_DP-GH023 | Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter |
| 90325 | pExchange_tdk_BT1311p-tetR-1 | Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702 |
| 90326 | pExchange_tdk_BT1311p-tetR-2 | Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694 |
| 90327 | pExchange_tdk_BT1311p-tetR-3 | Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 6193956 and 6193957 |
| 117727 | pNBU2_erm_P1T_DP-GH023 - NanoLuc | Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporter |
| 117728 | pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc | Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene |