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Relative and Absolute Quantification of Aberrant and Normal Splice Variants in HBB(IVSI-110 (G > A)) beta-Thalassemia.

Patsali P, Papasavva P, Christou S, Sitarou M, Antoniou MN, Lederer CW, Kleanthous M
Int J Mol Sci. 2020 Sep 11;21(18). pii: ijms21186671. doi: 10.3390/ijms21186671. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
87847pCR2.1_HBB_NpCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA
87848pCR2.1_HBB_ApCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNA

Antibodies from Article