Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms.
Zhang WW, Lypaczewski P, Matlashewski G
mSphere. 2017 Jan 18;2(1). pii: e00340-16. doi: 10.1128/mSphere.00340-16. eCollection 2017 Jan-Feb.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84290 | pLdCN | Express gRNA and Cas9 in Leishmania with Neomycin resistance |
| 84291 | pLdCH | Express gRNA and Cas9 in Leishmania with Hygromycin resistance |
| 84292 | pSPneogRNA241510+MT | Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously |