Designed proteins induce the formation of nanocage-containing extracellular vesicles.

Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King NP, Sundquist WI
Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
84511	pET29b-EPN01(∆L1+∆L2)-His	E.coli version of EPN01(∆L1+∆L2)
84510	pET29b-EPN01(∆M)-His	E.coli version of EPN01(∆M)
84509	pET29b-EPN01*-His	E.coli version of EPN01*
83375	pCMV-Myc-GFP	Expression of Myc tagged GFP
83374	pCMV-Myc-GFP-Vpr-RR	Expression of Myc tagged GFP-Vpr fusion protein
80423	pET29b-EPN-01*	For expression of EPN-01* in E. coli
80351	pEGFP-VSP4-E228Q	Expression of GFP-tagged, dominant negative VPS4 mutant
80124	pCMV-EPN-25 (1wa3)	I3-01p-myc-Late1-PH (1wa3)
80123	pCMV-EPN-18 (1wa3)	PH-4GS-I3-01-myc-Late1 (1wa3)
80122	pCMV-EPN-11 (1wa3)	Palm1-Late1-I3-01-myc (1wa3)