Designed proteins induce the formation of nanocage-containing extracellular vesicles.
Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King NP, Sundquist WI
Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 79916 | pCMV-EPN-01 | Myr1-12GS-I3-01-myc-Late1 |
| 79917 | pCMV-EPN-02 | Myr1_G2A-12GS-I3-01-myc-Late1 |
| 79918 | pCMV-EPN-03 | Myr2-12GS-I3-01-myc-Late1 |
| 79919 | pCMV-EPN-04 | Myr3-12GS-I3-01-myc-Late1 |
| 79920 | pCMV-EPN-05 | Myr4-12GS-I3-01-myc-Late1 |
| 79921 | pCMV-EPN-06 | Myr1-Late1-I3-01-myc |
| 79922 | pCMV-EPN-07 | Palm1-12GS-I3-01-myc-Late1 |
| 79923 | pCMV-EPN-08 | Palm2-12GS-I3-01-myc-Late1 |
| 79924 | pCMV-EPN-09 | Palm3-12GS-I3-01-myc-Late1 |
| 79925 | pCMV-EPN-10 | Palm4-12GS-I3-01-myc-Late1 |
| 79926 | pCMV-EPN-11 | Palm1-Late1-I3-01-myc |
| 79927 | pCMV-EPN-12 | I3-01p-myc-Late1-CaaX1 |
| 79928 | pCMV-EPN-13 | Late1-I3-01-myc-22GS-CaaX1 |
| 79929 | pCMV-EPN-14 | Late1-I3-01-myc-22GS-CaaX2 |
| 79930 | pCMV-EPN-15 | Late1-I3-01-myc-22GS-CaaX3 |
| 79931 | pCMV-EPN-16 | Late1-I3-01-myc-22GS-CaaX4 |
| 79932 | pCMV-EPN-17 | PH-4GS-I3-01-myc |
| 79933 | pCMV-EPN-18 | PH-4GS-I3-01-myc-Late1 |
| 79934 | pCMV-EPN-19 | Late1-I3-01-myc-PH |
| 79935 | pCMV-EPN-20 | Late2-4GS-I3-01-myc-PH |
| 79936 | pCMV-EPN-21 | Late11-I3-01-myc-PH |
| 79937 | pCMV-EPN-22 | Late12-I3-01-myc-PH |
| 79938 | pCMV-EPN-23 | Late1-PH-4GS-I3-01-myc |
| 79939 | pCMV-EPN-24 | PH-Late1-I3-01-myc |
| 79940 | pCMV-EPN-25 | I3-01p-myc-Late1-PH |
| 79941 | pCMV-EPN-26 | I3-01p-myc-10GS-PH-Late1 |
| 79942 | pCMV-EPN-27 | C1-10GS-I3-01-myc |
| 79943 | pCMV-EPN-28 | C1-10GS-I3-01-myc-Late1 |
| 79944 | pCMV-EPN-29 | Late1-I3-01-myc-10GS-C1 |
| 79945 | pCMV-EPN-30 | C2-22GS-I3-01-myc |
| 79946 | pCMV-EPN-31 | C2-22GS-I3-01-myc-Late1 |
| 79947 | pCMV-EPN-32 | Late1-I3-01-myc-22GS-C2 |
| 79948 | pCMV-EPN-33 | PX-10GS-I3-01-myc |
| 79949 | pCMV-EPN-34 | PX-10GS-I3-01-myc-Late1 |
| 79950 | pCMV-EPN-35 | Late1-I3-01-myc-4GS-PX |
| 79951 | pCMV-EPN-36 | Myr1-12GS-I3-01-myc-Late2 |
| 79952 | pCMV-EPN-37 | Myr1-12GS-I3-01-myc-Late3 |
| 79953 | pCMV-EPN-38 | Myr1-12GS-I3-01-myc-Late4 |
| 79954 | pCMV-EPN-39 | Myr1-12GS-I3-01-myc-22GS-Late1 (modified) |
| 79955 | pCMV-EPN-40 | Myr1-12GS-I3-01-myc-22GS-Late2 (modified) |
| 79956 | pCMV-EPN-41 | Myr1-12GS-I3-01-myc-22GS-Late3 |
| 79957 | pCMV-EPN-42 | Myr1-12GS-I3-01-myc-22GS-Late4 (modified) |
| 79958 | pCMV-EPN-43 | Myr1-12GS-I3-01-myc-22GS-Late5 (modified) |
| 79959 | pCMV-EPN-44 | Myr1-12GS-I3-01-myc-22GS-Late6 (modified) |
| 79960 | pCMV-EPN-45 | Myr1-12GS-I3-01-myc-22GS-Late7 (modified) |
| 79961 | pCMV-EPN-46 | Myr1-12GS-I3-01-myc-22GS-Late8 (modified) |
| 79962 | pCMV-EPN-47 | Myr1-12GS-I3-01-myc-22GS-Late9 (modified) |
| 79963 | pCMV-EPN-48 | Myr1-12GS-I3-01-myc-22GS-Late10 (modified) |
| 79964 | pCMV-EPN-49 | Myr1_G2A-4GS-O3-33-myc-Late1 |
| 79965 | pCMV-EPN-50 | Myr1-4GS-O3-33-myc-Late1 |
| 79966 | pCMV-EPN-51 | Late1-O3-33-myc-PH |
| 80038 | pCMV-I3-01-Myc | Expression of I3-01 |
| 80039 | pCMV-EPN-01* | Expression of EPN-01* |
| 80040 | pCMV-EPN-01*(ΔM) | Expression or G2A mutant of EPN-01* |
| 80041 | pCMV-EPN-01*(ΔI) | Expression of EPN-01 assembly mutant L33R |
| 80042 | pCMV-EPN-01*(ΔL1) | Expression of PTAP L domain mutant EPN-01* |
| 80043 | pCMV-EPN-01*(ΔL2) | Expression of YPxL L domain mutantd EPN-01* |
| 80044 | pCMV-EPN-01*(ΔL1+L2) | Expression of EPN-01* mutated in both L domains |
| 80045 | pCMV-EPN-01*-(ΔLF) | Expression of Vpr binding mutant EPN-01* |
| 80046 | pCMV-EPN-01 | expression of EPN-01 |
| 80047 | pCMV-EPN-01(ΔM) | Expression or G2A mutants of EPN-01 |
| 80048 | pCMV-EPN-01(ΔI) | Expression of EPN-01 assembly mutant L33R |
| 80049 | pCMV-EPN-01(ΔL1) | Expression of EPN-01 mutated in TSG101 binding site. |
| 80050 | pCMV-EPN-01(ΔL2) | Expression of EPN-01 mutated in ALIX binding site. |
| 80051 | pCMV-EPN-01(ΔL1+L2) | Expression of EPN-01 mutated in both L domains |
| 80052 | pCMV-EPN-01-(ΔLF) | Expression of Vpr binding mutant EPN-01 |
| 80053 | pMM310-Myc | Expression of a Myc tagged version of the BlaM-Vpr fusion protein |
| 80054 | pCMV-VSV-G-Myc | Expression of a Myc tagged VSV-G protein |
| 80055 | pCMV-VSV-G(P127D)-Myc | Expression of a Myc tagged VSV-G protein |
| 80104 | pCMV-EPN-03 (G2A) | Myr2(G2A)-12GS-I3-01-myc-Late1 |
| 80105 | pCMV-EPN-07 (2AA3) | Palm1(2AA3)-12GS-I3-01-myc-Late1 |
| 80106 | pCMV-EPN-08 (2AA3) | Palm2(2AA3)-12GS-I3-01-myc-Late1 |
| 80107 | pCMV-EPN18 (R40A) | PH(R40A)-4GS-I3-01-myc-Late1 |
| 80108 | pCMV-EPN51 (L1L2) | Late1(delta)-O3-33-myc-PH |
| 80109 | pCMV-EPN51 (R40A) | Late1-O3-33-myc-PH(R40A) |
| 80110 | pCMV-EPN51 (A167R) | Late1-O3-33(A167R)-myc-PH |
| 80111 | pCMV-EPN11 (L1L2) | Palm1-Late1(delta)-I3-01-myc |
| 80112 | pCMV-EPN20 (deltaL) | Late2(delta)-4GS-I3-01-myc-PH |
| 80113 | pCMV-EPN23 (L1L2) | Late1(delta)-PH-4GS-I3-01-myc |
| 80114 | pCMV-EPN24 (L1L2) | PH-Late1(delta)-I3-01-myc |
| 80115 | pCMV-EPN25 (L1L2) | I3-01p-myc-Late1(delta)-PH |
| 80116 | pCMV-EPN-36 (deltaL) | Myr1-12GS-I3-01-myc-Late2(delta) |
| 80117 | pCMV-EPN-37 (deltaL) | Myr1-12GS-I3-01-myc-Late3(delta) |
| 80118 | pCMV-EPN-41 (deltaL) | Myr1-12GS-I3-01-myc-22GS-Late3(delta) |
| 80119 | pCMV-EPN-01 (1wa3) | Myr1-12GS-I3-01-myc-Late1 (1wa3) |
| 80120 | pCMV-EPN-03 (1wa3) | Myr2-12GS-I3-01-myc-Late1 (1wa3) |
| 80121 | pCMV-EPN-07 (1wa3) | Palm1-12GS-I3-01-myc-Late1 (1wa3) |
| 80122 | pCMV-EPN-11 (1wa3) | Palm1-Late1-I3-01-myc (1wa3) |
| 80123 | pCMV-EPN-18 (1wa3) | PH-4GS-I3-01-myc-Late1 (1wa3) |
| 80124 | pCMV-EPN-25 (1wa3) | I3-01p-myc-Late1-PH (1wa3) |
| 80351 | pEGFP-VSP4-E228Q | Expression of GFP-tagged, dominant negative VPS4 mutant |
| 80423 | pET29b-EPN-01* | For expression of EPN-01* in E. coli |
| 83374 | pCMV-Myc-GFP-Vpr-RR | Expression of Myc tagged GFP-Vpr fusion protein |
| 83375 | pCMV-Myc-GFP | Expression of Myc tagged GFP |
| 84509 | pET29b-EPN01*-His | E.coli version of EPN01* |
| 84510 | pET29b-EPN01(∆M)-His | E.coli version of EPN01(∆M) |
| 84511 | pET29b-EPN01(∆L1+∆L2)-His | E.coli version of EPN01(∆L1+∆L2) |