mScarlet: a bright monomeric red fluorescent protein for cellular imaging.
Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85042 | pmScarlet_C1 | Mammalian expression of mScarlet |
| 85043 | pmScarlet-H_C1 | Mammalian expression of mScarlet-H |
| 85044 | pmScarlet-i_C1 | Mammalian expression of mScarlet-i |
| 85045 | pmScarlet_alphaTubulin_C1 | In vivo visualization of microtubules (can be used for colocalization studies) |
| 85046 | pmScarlet-H_alphaTubulin_C1 | In vivo visualization of microtubules (can be used for colocalization studies) |
| 85047 | pmScarlet-i_alphaTubulin_C1 | In vivo visualization of microtubules (can be used for colocalization studies) |
| 85048 | pmScarlet_Giantin_C1 | In vivo visualization of the Golgi apparatus (can be used for colocalization studies) |
| 85049 | pmScarlet-H_Giantin_C1 | In vivo visualization of the Golgi apparatus (can be used for colocalization studies) |
| 85050 | pmScarlet-i_Giantin_C1 | In vivo visualization of the Golgi apparatus (can be used for colocalization studies) |
| 85051 | pmScarlet_H2A_C1 | In vivo visualization of the nucleus (can be used for colocalization studies) |
| 85052 | pmScarlet-H_H2A_C1 | In vivo visualization of the nucleus (can be used for colocalization studies) |
| 85053 | pmScarlet-i_H2A_C1 | In vivo visualization of the nucleus (can be used for colocalization studies) |
| 85054 | pLifeAct_mScarlet_N1 | In vivo visualization of actin cytoskeleton (can be used for colocalization studies) |
| 85055 | pLifeAct_mScarlet-H_N1 | In vivo visualization of actin cytoskeleton (can be used for colocalization studies) |
| 85056 | pLifeAct_mScarlet-i_N1 | In vivo visualization of actin cytoskeleton (can be used for colocalization studies) |
| 85057 | pMTS_mScarlet_N1 | In vivo visualization of the mitochondria (can be used for colocalization studies) |
| 85058 | pMTS_mScarlet-H_N1 | In vivo visualization of the mitochondria (can be used for colocalization studies) |
| 85059 | pMTS_mScarlet-i_N1 | In vivo visualization of the mitochondria (can be used for colocalization studies) |
| 85060 | pmScarlet_NES_C1 | In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies) |
| 85061 | pmScarlet-H_NES_C1 | In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies) |
| 85062 | pmScarlet-i_NES_C1 | In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies) |
| 85063 | pmScarlet_peroxisome_C1 | In vivo visualization of the peroxisome (can be used for colocalization studies) |
| 85064 | pmScarlet-H_peroxisome_C1 | In vivo visualization of the peroxisome (can be used for colocalization studies) |
| 85065 | pmScarlet-I_peroxisome_C1 | In vivo visualization of the peroxisome (can be used for colocalization studies) |
| 85066 | pCytERM_mScarlet_N1 | In vivo visualization of the ER (can be used for colocalization studies and the OSER assay) |
| 85067 | pCytERM_mScarlet-H_N1 | In vivo visualization of the ER (can be used for colocalization studies and the OSER assay) |
| 85068 | pCytERM_mScarlet-i_N1 | In vivo visualization of the ER (can be used for colocalization studies and the OSER assay) |
| 85069 | PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1 | Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2) |
| 85070 | PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1 | Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2) |
| 85071 | pTriEx-RhoA-wt_mScarlet-i_SGFP2 | Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity |
| 85072 | pTriEx-RhoA-Q63L_mScarlet-i_SGFP2 | Green/red FRET biosensor for RhoA in the GTP locked constitutively active state |
| 98834 | CytERM-dTomato | In vivo visualization of the ER (can be used for colocalization studies and the OSER assay) |
| 110623 | PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1 | It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio. |