VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy.

Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, Poehler R, Dressler A, Fengler S, Arhzaouy K, Lux V, Ehrmann M, Weihl CC, Meyer H
EMBO J. 2016 Oct 17. pii: e201695148. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
85662	pmCherry-Gal3	Fluorescent reporter for lysosomal damage
85663	mCherry-YOD1	Expression of human YOD1 with N-terminal mCherry tag
85664	pEGFP-YOD1	Expression of human YOD1 with N-terminal GFP tag
85666	pEGFP-YOD1-C160S	Expression of human YOD1 C160S mutant with N-terminal GFP tag
85667	pEGFP-YOD1-MutS2	Expression of human YOD1 I292Q V295Q mutant with N-terminal GFP tag
85668	pEGFP-YOD1-MutS2-C160S	Expression of human YOD1 I292Q V295Q C160S mutant with N-terminal GFP tag
85669	pEGFP-PLAA	Expression of PLAA with N-terminal GFP tag
85670	pEGFP-p97	Expression of p97 with N-terminal GFP tag
85671	pEGFP-VCPIP1	Expression of VCPIP1 with N-terminal GFP tag
85672	pEGFP-p97-E578Q	Expression of human p97 E578Q mutant with N-terminal GFP tag
86464	UBXD1-GFP	Expression of human UBXD1 with C-terminal GFP tag