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PurposeExpression of p97 with N-terminal GFP tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85670 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namep97
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Alt nameVCP
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Alt namevalosin-containing protein
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SpeciesR. norvegicus (rat)
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GenBank IDNM_053864.2
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Entrez GeneVcp
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bycloned from pcDNA5-p97-wt-myc-strep (Ritz et al. 2011, NCB)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS results found the addition of amino acids GSTGSR at the C-terminus of the p97 translation
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-p97 was a gift from Hemmo Meyer (Addgene plasmid # 85670 ; http://n2t.net/addgene:85670 ; RRID:Addgene_85670) -
For your References section:
VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy. Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, Poehler R, Dressler A, Fengler S, Arhzaouy K, Lux V, Ehrmann M, Weihl CC, Meyer H. EMBO J. 2016 Oct 17. pii: e201695148. 10.15252/embj.201695148 PubMed 27753622