An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting.
Cao J, Wu L, Zhang SM, Lu M, Cheung WK, Cai W, Gale M, Xu Q, Yan Q
Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85400 | Lenti-iCas9-neo | Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence |
| 85401 | Lenti-multi-Guide | Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo |
| 85402 | Lenti-multi-CRISPR | Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression |
| 85745 | Lenti-entry-puro | Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly. |