Ligand Modulates Cross-Coupling between Riboswitch Folding and Transcriptional Pausing.
Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch I, Walter NG
Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 10.1016/j.molcel.2018.08.046.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84718 | pVR | E. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilis |
| 84719 | pVR2 | E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription |
| 84720 | pVR2-m1 | pVR2 but with a C to U mutation in the riboswitch aptamer |
| 84721 | pVR2-m2 | pVR2 but with a G to U mutation in the riboswitch aptamer |
| 84722 | pVR2-m3 | pVR2 but with a C to G mutation in the riboswitch aptamer |
| 84723 | pVR2-m4 | pVR2 but with G to U and A to C mutations following the riboswitch aptamer |
| 84724 | pVR2-m5 | pVR2 but with an insertion of 1 U immediately following the riboswitch aptamer |
| 84725 | pVR2-m6 | pVR2 but with an insertion of 2 U's immediately following the riboswitch aptamer |
| 84726 | pVR2-m7 | pVR2 but with a G to U mutation immediately following the riboswitch aptamer |
| 84727 | pVR2-m8 | pVR2 but with two G to U mutations immediately following the riboswitch aptamer |