Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.
Goldenzweig A, Goldsmith M, Hill SE, Gertman O, Laurino P, Ashani Y, Dym O, Unger T, Albeck S, Prilusky J, Lieberman RL, Aharoni A, Silman I, Sussman JL, Tawfik DS, Fleishman SJ
Mol Cell. 2016 Jul 21;63(2):337-46. doi: 10.1016/j.molcel.2016.06.012. Epub 2016 Jul 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83916 | pET32bplus_trxAChEwt | Encodes an E. coli codon optimized version of wild type human acetylcholinesterase |
| 83917 | pET32bplus_trx_dAChE4 | Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli |