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Trapping mammalian protein complexes in viral particles.

Eyckerman S, Titeca K, Van Quickelberghe E, Cloots E, Verhee A, Samyn N, De Ceuninck L, Timmerman E, De Sutter D, Lievens S, Van Calenbergh S, Gevaert K, Tavernier J
Nat Commun. 2016 Apr 28;7:11416. doi: 10.1038/ncomms11416. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
80604pMET7-GAG-HRASMammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it
80605pMET7-GAG-EGFPMammalian expression vector that expresses HIV-1 GAG with EGFP fused to it
80606pcDNA3-FLAG-VSVGMammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the protein
194634pMET7-GAG-GWGateway destination vector for mammalian expression of Virotrap bait constructs
194635pMET7-GAG-eDHFRMammalian expression of the eDHFR control bait in Virotrap

Antibodies from Article