Synthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002.
Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF
ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. Epub 2014 Sep 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 82019 | pACSA_Pcpt_YFP | pALM34; control for YFP expression from cyanobacterial phycocyanin truncated promoter |
| 82020 | pACSA_PcptOO_YFP_PMB2__LacIWF | cLac94; pALM94; IPTG inducible system |
| 82021 | pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF | cLac143; pALM143; IPTG inducible system |
| 82022 | pALM174 | an updated version of cLac94 (pACSA_PcptOO_YFP_PMB2__LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression |
| 82023 | pALM179 | an updated version of cLac143 (pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression |