Metabolism. AMP-activated protein kinase mediates mitochondrial fission in response to energy stress.
Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74374 | pX462-hPRKAA1-gRNA_A | gRNA_A to knockout human AMPK alpha 1 using Cas9n |
| 74375 | pX462-hPRKAA1-gRNA_B | gRNA_B to knockout human AMPK alpha 1 using Cas9n |
| 74376 | pX462-hPRKAA2-gRNA_A | gRNA_A to knockout human AMPK alpha 2 using Cas9n. |
| 74377 | pX462-hPRKAA2-gRNA_B | gRNA_B to knockout human AMPK alpha 2 using Cas9n |
| 74378 | pCDNA3 Flag MFF iso5 WT | Flag tagged human MFF isoform 5 |
| 74379 | pLenti.PGK.blast-Flag-MFF-WT | Lentiviral expression vector containing flag tagged WT MFF isoform 5 |
| 74380 | pLenti.PGK.blast-Flag-MFF-S155,172A | Lentiviral expression vector containing flag tagged S155,172A MFF isoform 5 mutant |
| 74381 | pCDNA3 Flag MFF iso5 S155A | Flag tagged human S155A MFF isoform 5 mutant |
| 74382 | pCDNA3 Flag MFF iso5 S172A | Flag tagged human S172A MFF isoform 5 mutant |
| 74383 | pCDNA3 Flag MFF iso5 S155,172A | Flag tagged human S155,172A MFF isoform 5 mutant |
| 74384 | pQCXIB Flag MFF WT | Retroviral expression vector containing Flag tagged WT MFF isoform 5 |
| 74385 | pQCXIB Flag MFF S155A | Retroviral expression vector containing Flag tagged S155A MFF isoform 5 mutant |
| 74386 | pQCXIB Flag MFF S172A | Retroviral expression vector containing Flag tagged S172A MFF isoform 5 mutant |
| 74387 | pQCXIB Flag MFF S155,172A | Retroviral expression vector containing Flag tagged S155,172A MFF isoform 5 mutant |
| 74388 | pcDNA3 Flag MFF iso1 WT | Flag tagged human MFF isoform 1 |
| 74389 | pcDNA3 Flag MFF iso2 WT | Flag tagged human MFF isoform 2 |
| 74390 | pcDNA3 Flag MFF iso4 WT | Flag tagged human MFF isoform 4 |
| 74391 | pcDNA3 Flag MFF iso1 S172A | Flag tagged human S172A MFF isoform 1 mutant |
| 74392 | pcDNA3 Flag MFF iso2 S172A | Flag tagged human S172A MFF isoform 2 mutant |
| 74393 | pcDNA3 Flag MFF iso4 S172A | Flag tagged human S172A MFF isoform 4 mutant |
| 74442 | pLenti.PGK.blast-Flag-MFF-S155,172D | Lentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutant |
| 74443 | pLenti.PGK.blast-Flag-MFF-S155,172E | Lentiviral expression vector containing flag tagged S155,172E MFF isoform 5 mutant |
| 74444 | pLenti.PGK.blast-Renilla_Luciferase | Control lentiviral vector expressing Renilla luciferase |
| 74446 | pLenti.CMV/TO_PRKAA1 | Lentiviral expression vector encoding untagged WT human AMPK alpha 1 |
| 74447 | pLenti.CMV/TO_PRKAA2 | Lentiviral expression vector encoding untagged WT human AMPK alpha 2 |