Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73850 | pAWp28 | pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI} |
| 73851 | pAWp9 | pFUGW-UBCp-RFP-CMVp-GFP-{BamHI+EcoRI} |
| 73852 | pAWp9-1 | pFUGW-UBCp-RFP-CMVp-GFP-U6p-GFP-sg1 |
| 73853 | pAWp9-2 | pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1 |
| 73854 | pAWp9-3 | pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2 |
| 73855 | pAWp9-4 | pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1 |
| 73856 | pAWp9-5 | pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1 |
| 73857 | pAWp30 | pFUGW-EFSp-Cas9-P2A-Zeo |