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Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability.

Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, Jessell TM, Losonczy A
Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 10.1016/j.neuron.2016.01.004. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
73460RabV CVS-N2c(deltaG)-dsRedExpresses dsRed for monosynaptic circuit mapping
73461RabV CVS-N2c(deltaG)-EGFPExpresses EGFP for monosynaptic circuit mapping
73462RabV CVS-N2c(deltaG)-tdTomatoExpresses tdTomato for monosynaptic circuit mapping
73464RabV CVS-N2c(deltaG)-mCherryExpresses mCherry for monosynaptic circuit mapping
73465RabV CVS-N2c(deltaG)-hChR2-YFPExpresses channelrhodopsin fused to YFP for monosynaptic circuit mapping and optogenetic activation
73466RabV CVS-N2c(deltaG)-GCaMP6fExpresses GCaMP6f for calcium imaging
73467RabV CVS-N2c(deltaG)-APEX2-VenusExpresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.
73471RabV CVS-N2c(deltaG)-mCherry-P2A-FlpOExpresses mCherry and FlpO for monosynaptic circuit mapping and genetic manipulation
73472RabV CVS-N2c(deltaG)-mCherry-P2A-CreExpresses mCherry and Cre for monosynaptic circuit mapping and genetic manipulation
73473RabV CVS-N2c(deltaG)-FlpOExpresses FlpO for circuit mapping and genetic manipulation
73474RabV CVS-N2c(deltaG)-CreExpresses Cre for circuit mapping and genetic manipulation
73475pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G)Encodes the G protein to enable transsynaptic RAV infection
73476DIO-EF1a-FLEX-H2B-GFP-P2A-N2c(G)Encodes the G protein to enable transsynaptic RAV infection
73477pAM-CAGGS-FLEX-H2B-3XHA-P2A-N2c(G)Encodes the G protein to enable transsynaptic RAV infection
73478DIO-EF1a-FLEX-H2B-3XHA-P2A-N2c(G)Encodes the G protein to enable transsynaptic RAV infection
73481pCAGGS-N2c(G)N2c Glycoprotein expression vector
73492RabV CVS N2c(deltaG)-synaptophysin-GFPExpresses EGFP for monosynaptic circuit mapping at the synapse

Antibodies from Article