CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
Iverson SV, Haddock TL, Beal J, Densmore DM
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65980 | J23100_AB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [A:J23100:B] |
| 65981 | J23100_EB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [E:J23100:B] |
| 65982 | J23100_FB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [F:J23100:B] |
| 65983 | J23100_GB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B] |
| 65984 | J23102_AB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [A:J23102:B] |
| 65985 | J23102_EB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [E:J23102:B] |
| 65986 | J23102_FB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [F:J23102:B] |
| 65987 | J23102_GB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23102:B] |
| 65988 | J23103_AB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [A:J23103:B] |
| 65989 | J23103_EB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [E:J23103:B] |
| 65990 | J23103_FB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [F:J23103:B] |
| 65991 | J23103_GB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B] |
| 65992 | J23106_AB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [A:J23106:B] |
| 65993 | J23106_EB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [E:J23106:B] |
| 65994 | J23106_FB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [F:J23106:B] |
| 65995 | J23106_GB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23106:B] |
| 65996 | J23107_AB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [A:J23107:B] |
| 65997 | J23107_EB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [E:J23107:B] |
| 65998 | J23107_FB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [F:J23107:B] |
| 65999 | J23107_GB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B] |
| 66000 | J23116_AB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [A:J23116:B] |
| 66001 | J23116_EB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [E:J23116:B] |
| 66002 | J23116_FB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [F:J23116:B] |
| 66003 | J23116_GB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B] |
| 66004 | R0010 (pLacI)_AB | MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B] |
| 66005 | R0010 (pLacI)_EB | MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [E:R0010:B] |
| 66006 | R0010 (pLacI)_FB | MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [F:R0010:B] |
| 66007 | R0010 (pLacI)_GB | MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [G:R0010:B] |
| 66008 | R0040 (pTet)_AB | MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [A:R0040:B] |
| 66009 | R0040 (pTet)_EB | MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [E:R0040:B] |
| 66010 | R0040 (pTet)_FB | MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [F:R0040:B] |
| 66011 | R0040 (pTet)_GB | MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [G:R0040:B] |
| 66012 | R0063 (pLuxR-pR)_AB | MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [A:R0063:B] |
| 66013 | R0063 (pLuxR-pR)_EB | MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [E:R0063:B] |
| 66014 | R0063 (pLuxR-pR)_FB | MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [F:R0063:B] |
| 66015 | R0063 (pLuxR-pR)_GB | MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [G:R0063:B] |
| 66016 | I13453 (pBAD)_AB | MoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [A:I13453:B] |
| 66017 | I13453 (pBAD)_EB | MoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [E:I13453:B] |
| 66018 | I13453 (pBAD)_FB | MoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [F:I13453:B] |
| 66019 | I13453 (pBAD)_GB | MoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [G:I13453:B] |
| 66020 | B0032m_BC | MoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C] |
| 66021 | B0033m_BC | MoClo Basic Part: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. [B:B0033m:C] |
| 66022 | B0034m_BC | MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. [B:B0034m:C] |
| 66023 | BCD12_BC | MoClo Basic Part: RBS - BiCistronic Design, medium strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD12:C] |
| 66024 | BCD2_BC | MoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD2:C] |
| 66025 | BCD8_BC | MoClo Basic Part: RBS - BiCistronic Design, low strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD8:C] |
| 66026 | C0012m (lacI)_CD | MoClo Basic Part: CDS - Controller protein, lacI repressor (in concert with CAP, represses pLacI, R0010). Modified to fix illegal site. [C:C0012m:D] |
| 66027 | C0040 (tetR)_CD | MoClo Basic Part: CDS - Controller protein, tetR repressor (represses pTet, C0040. can be inhibited by tetracyclin or aTc) [C:C0040:D] |
| 66028 | C0062 (luxR)_CD | MoClo Basic Part: CDS - Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062) [C:C0062:D] |
| 66029 | C0080 (araC)_CD | MoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D] |
| 66030 | cre_CD | MoClo Basic Part: CDS - cre recombinase (recombines DNA at loxP sites) [C:cre:D] |
| 66031 | E0030 (YFP)_CD | MoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D] |
| 66032 | E0040m (GFP)_CD | MoClo Basic Part: CDS - Fluorescent protein. Green. Modified from Bba_E0040 to fix illegal site. [C:E0040m:D] |
| 66033 | E1010m (RFP)_CD | MoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D] |
| 66034 | eBFP2 (eBFP2)_CD | MoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D] |
| 66035 | B0015_DE | MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:E] |
| 66036 | B0015_DF | MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:F] |
| 66037 | B0015_DG | MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:G] |
| 66038 | B0015_DH | MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:H] |
| 66039 | DVA_AB | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:B] |
| 66040 | DVA_AE | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:E] |
| 66041 | DVA_AF | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:F] |
| 66042 | DVA_AG | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:G] |
| 66043 | DVA_AH | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:H] |
| 66044 | DVA_BC | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [B:LacZa:C] |
| 66045 | DVA_CD | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [C:LacZa:D] |
| 66046 | DVA_DE | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:E] |
| 66047 | DVA_DF | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:F] |
| 66048 | DVA_DG | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:G] |
| 66049 | DVA_DH | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:H] |
| 66050 | DVA_EB | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:B] |
| 66051 | DVA_EF | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:F] |
| 66052 | DVA_EG | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:G] |
| 66053 | DVA_EH | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:H] |
| 66054 | DVA_FB | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [F:LacZa:B] |
| 66055 | DVA_GB | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [G:LacZa:B] |
| 66056 | DVA | MoClo Destination Vector: Empty vector for creating new DVA. Cut with SpeI and insert amplified LacZa (based on pSB1A2) |
| 66057 | pJ02B2Gm_AE | MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E] |
| 66058 | pJ02B2Gm_EF | MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F] |
| 66059 | pJ02B2Rm_AE | MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E] |
| 66060 | pJ02B2Rm_EF | MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F] |
| 66061 | pJ02B2Gm:Rm_AF | MoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F] |
| 66062 | pJ02B2Rm:Gm_AF | MoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F] |
| 66063 | pJ02B2Gm_AE | MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E] |
| 66064 | pJ02B2Gm_EF | MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F] |
| 66065 | pJ02B2Rm_AE | MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E] |
| 66066 | pJ02B2Rm_EF | MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F] |
| 66068 | DVK_AF | MoClo Destination Vector: (based on pSB1K3) [A:LacZa:F] |
| 66070 | DVK_FG | MoClo Destination Vector: (based on pSB1K3) [F:LacZa:G] |
| 66071 | DVK_GH | MoClo Destination Vector: (based on pSB1K3) [G:LacZa:H] |
| 66072 | DVK | MoClo Destination Vector: Empty vector for creating new DVK. Cut with SpeI and insert amplified LacZa (based on pSB1K3) |