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Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing.

Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov K, Nevo Y, Eyras E, Sperling R, Stamm S
Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 10.1073/pnas.1519292113. Epub 2016 Mar 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
73063pCMV14_NOP58expression clone for human NOP58 with C-terminal 3xFLAG-tag
73065pCMV14_NHP2L1(15.5kD)expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag
73066pcDNA3.1_SNORD27hexpression clone for human SNORD27 (C/D box snoRNA U27)
73067pcDNA3.1_SNORD27h_2ntmutexpression clone for human mutated SNORD27 (C/D box snoRNA U27)
73068pcDNA3.1_SNORD27h_115mutexpression clone for human mutated SNORD27 (C/D box snoRNA U27)
73069pCR4_SNORD27 probeantisense probe for detection of human SNORD27
73070pcDNA3.1_SNORD2 probeantisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)
73071pcDNA3.1_SNORD60 probeantisense probe for detection of human SNORD60 (C/D box snoRNA U60)
73072pET21d_SNORD78_probeantisense probe for detection of human SNORD78 (C/D box snoRNA U78)
73073pcDNA3.1_hE2F7mghuman E2F7 minigene (exons 11,12,13/introns cassette)
73074pcDNA3.1_hE2F7mg_2ntmuthuman E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site
73075pcDNA3.1_hE2F7mg_6ntmuthuman E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site
73076pcDNA3.1_hE2F7mg_10ntmuthuman E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site
73077pcDNA3.1_hE2F7mg_115muthuman E2F7 minigene (exons 11,12,13/introns cassette) with inserted SNORD115 binding site
73078pcDNA3.1_hE2F7mg_PP7-taghuman E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-end

Antibodies from Article