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SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance.

Kim D, Fiske BP, Birsoy K, Freinkman E, Kami K, Possemato RL, Chudnovsky Y, Pacold ME, Chen WW, Cantor JR, Shelton LM, Gui DY, Kwon M, Ramkissoon SH, Ligon KL, Kang SW, Snuderl M, Vander Heiden MG, Sabatini DM
Nature. 2015 Apr 16;520(7547):363-7. doi: 10.1038/nature14363. Epub 2015 Apr 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
72880SHMT2-RESoverexpression
72881mGLDCoverexpression
72882GLDC-RESoverexpression
72883shGLDC-DOXdox inducible knockdown
72885sgGCAT_2CRISPR-Cas9 induced gene disruption
72886sgGLDC_1CRISPR-Cas9 induced gene disruption
72887sgGLDC_2CRISPR-Cas9 induced gene disruption

Antibodies from Article