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Neuronal ensembles sufficient for recovery sleep and the sedative actions of alpha2 adrenergic agonists.

Zhang Z, Ferretti V, Guntan I, Moro A, Steinberg EA, Ye Z, Zecharia AY, Yu X, Vyssotski AL, Brickley SG, Yustos R, Pillidge ZE, Harding EC, Wisden W, Franks NP
Nat Neurosci. 2015 Apr;18(4):553-61. doi: 10.1038/nn.3957. Epub 2015 Feb 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
66794pAAV-cFos-tTA-pAExpresses TetActivator protein under the control of the c-fos promoter for use in TetTaging
66795pAAV-PTRE-tight-hM3Dq-mCherryExpresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging
67879AAV-actin prom-dsRed-scramble shRNAAAV vector to express dsRed sh RNA (scramble)
67880AAV-actin prom-dsRed-adra2a.1227.shRNAexpresses dsRed and shRNA to knock down adra2a receptors

Antibodies from Article