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A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly.

Lee ME, DeLoache WC, Cervantes B, Dueber JE
ACS Synth Biol. 2015 Apr 14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
65108pYTK001Part Plasmid Entry Vector to be used in the Dueber YTK system.
65109pYTK002Encodes ConLS as a Type 1 part to be used in the Dueber YTK system
65110pYTK003Encodes ConL1 as a Type 1 part to be used in the Dueber YTK system
65111pYTK004Encodes ConL2 as a Type 1 part to be used in the Dueber YTK system
65112pYTK005Encodes ConL3 as a Type 1 part to be used in the Dueber YTK system
65113pYTK006Encodes ConL4 as a Type 1 part to be used in the Dueber YTK system
65114pYTK007Encodes ConL5 as a Type 1 part to be used in the Dueber YTK system
65115pYTK008Encodes ConLS' as a Type 1 part to be used in the Dueber YTK system
65116pYTK009Encodes pTDH3 as a Type 2 part to be used in the Dueber YTK system
65117pYTK010Encodes pCCW12 as a Type 2 part to be used in the Dueber YTK system
65118pYTK011Encodes pPGK1 as a Type 2 part to be used in the Dueber YTK system
65119pYTK012Encodes pHHF2 as a Type 2 part to be used in the Dueber YTK system
65120pYTK013Encodes pTEF1 as a Type 2 part to be used in the Dueber YTK system
65121pYTK014Encodes pTEF2 as a Type 2 part to be used in the Dueber YTK system
65122pYTK015Encodes pHHF1 as a Type 2 part to be used in the Dueber YTK system
65123pYTK016Encodes pHTB2 as a Type 2 part to be used in the Dueber YTK system
65124pYTK017Encodes pRPL18B as a Type 2 part to be used in the Dueber YTK system
65125pYTK018Encodes pALD6 as a Type 2 part to be used in the Dueber YTK system
65126pYTK019Encodes pPAB1 as a Type 2 part to be used in the Dueber YTK system
65127pYTK020Encodes pRET2 as a Type 2 part to be used in the Dueber YTK system
65128pYTK021Encodes pRNR1 as a Type 2 part to be used in the Dueber YTK system
65129pYTK022Encodes pSAC6 as a Type 2 part to be used in the Dueber YTK system
65130pYTK023Encodes pRNR2 as a Type 2 part to be used in the Dueber YTK system
65131pYTK024Encodes pPOP6 as a Type 2 part to be used in the Dueber YTK system
65132pYTK025Encodes pRAD27 as a Type 2 part to be used in the Dueber YTK system
65133pYTK026Encodes pPSP2 as a Type 2 part to be used in the Dueber YTK system
65134pYTK027Encodes pREV1 as a Type 2 part to be used in the Dueber YTK system
65135pYTK028Encodes pMFA1 as a Type 2 part to be used in the Dueber YTK system
65136pYTK029Encodes pMF_2 as a Type 2 part to be used in the Dueber YTK system
65137pYTK030Encodes pGAL1 as a Type 2 part to be used in the Dueber YTK system
65138pYTK031Encodes pCUP1 as a Type 2 part to be used in the Dueber YTK system
65139pYTK032Encodes mTurquoise2 as a Type 3 part to be used in the Dueber YTK system
65140pYTK033Encodes Venus as a Type 3 part to be used in the Dueber YTK system
65141pYTK034Encodes mRuby2 as a Type 3 part to be used in the Dueber YTK system
65142pYTK035Encodes I-SceI (ORF) as a Type 3 part to be used in the Dueber YTK system
65143pYTK036Encodes Cas9 as a Type 3 part to be used in the Dueber YTK system
65144pYTK037Encodes mTurquoise2 as a Type 3a part to be used in the Dueber YTK system
65145pYTK038Encodes Venus as a Type 3a part to be used in the Dueber YTK system
65146pYTK039Encodes mRuby2 as a Type 3a part to be used in the Dueber YTK system
65147pYTK040Encodes 6XHis_3XFlag as a Type 3a part to be used in the Dueber YTK system
65148pYTK041Encodes Ubi-M as a Type 3a part to be used in the Dueber YTK system
65149pYTK042Encodes Ubi-Y as a Type 3a part to be used in the Dueber YTK system
65150pYTK043Encodes Ubi-R as a Type 3a part to be used in the Dueber YTK system
65151pYTK044Encodes mTurquoise2 as a Type 3b part to be used in the Dueber YTK system
65152pYTK045Encodes Venus as a Type 3b part to be used in the Dueber YTK system
65153pYTK046Encodes mRuby2 as a Type 3b part to be used in the Dueber YTK system
65154pYTK047Encodes GFP dropout as a Type 234r part to be used in the Dueber YTK system
65155pYTK048Encodes Spacer as a Type 234 part to be used in the Dueber YTK system
65156pYTK049Encodes I-SceI recognition site as a Type 234 part to be used in the Dueber YTK system
65157pYTK050Encodes sgRNA Dropout as a Type 234 part to be used in the Dueber YTK system
65158pYTK051Encodes tENO1 as a Type 4 part to be used in the Dueber YTK system
65159pYTK052Encodes tSSA1 as a Type 4 part to be used in the Dueber YTK system
65160pYTK053Encodes tADH1 as a Type 4 part to be used in the Dueber YTK system
65161pYTK054Encodes tPGK1 as a Type 4 part to be used in the Dueber YTK system
65162pYTK055Encodes tENO2 as a Type 4 part to be used in the Dueber YTK system
65163pYTK056Encodes tTDH1 as a Type 4 part to be used in the Dueber YTK system
65164pYTK057Encodes mTurquoise2 as a Type 4a part to be used in the Dueber YTK system
65165pYTK058Encodes Venus as a Type 4a part to be used in the Dueber YTK system
65166pYTK059Encodes mRuby2 as a Type 4a part to be used in the Dueber YTK system
65167pYTK060Encodes 6XHis_3XFlag as a Type 4a part to be used in the Dueber YTK system
65168pYTK061Encodes tENO1 as a Type 4b part to be used in the Dueber YTK system
65169pYTK062Encodes tSSA1 as a Type 4b part to be used in the Dueber YTK system
65170pYTK063Encodes tADH1 as a Type 4b part to be used in the Dueber YTK system
65171pYTK064Encodes tPGK1 as a Type 4b part to be used in the Dueber YTK system
65172pYTK065Encodes tENO2 as a Type 4b part to be used in the Dueber YTK system
65173pYTK066Encodes tTDH1 as a Type 4b part to be used in the Dueber YTK system
65174pYTK067Encodes ConR1 as a Type 5 part to be used in the Dueber YTK system
65175pYTK068Encodes ConR2 as a Type 5 part to be used in the Dueber YTK system
65176pYTK069Encodes ConR3 as a Type 5 part to be used in the Dueber YTK system
65177pYTK070Encodes ConR4 as a Type 5 part to be used in the Dueber YTK system
65178pYTK071Encodes ConR5 as a Type 5 part to be used in the Dueber YTK system
65179pYTK072Encodes ConRE as a Type 5 part to be used in the Dueber YTK system
65180pYTK073Encodes ConRE' as a Type 5 part to be used in the Dueber YTK system
65181pYTK074Encodes URA3 as a Type 6 part to be used in the Dueber YTK system
65182pYTK075Encodes LEU2 as a Type 6 part to be used in the Dueber YTK system
65183pYTK076Encodes HIS3 as a Type 6 part to be used in the Dueber YTK system
65184pYTK077Encodes KanamycinR as a Type 6 part to be used in the Dueber YTK system
65185pYTK078Encodes NourseothricinR as a Type 6 part to be used in the Dueber YTK system
65186pYTK079Encodes HygromycinR as a Type 6 part to be used in the Dueber YTK system
65187pYTK080Encodes ZeocinR as a Type 6 part to be used in the Dueber YTK system
65188pYTK081Encodes CEN6/ARS4 as a Type 7 part to be used in the Dueber YTK system
65189pYTK082Encodes 2micron as a Type 7 part to be used in the Dueber YTK system
65190pYTK083Encodes AmpR-ColE1 as a Type 8 part to be used in the Dueber YTK system
65191pYTK084Encodes KanR-ColE1 as a Type 8 part to be used in the Dueber YTK system
65192pYTK085Encodes SpecR-ColE1 as a Type 8 part to be used in the Dueber YTK system
65193pYTK086Encodes URA3 3' Homology as a Type 7 part to be used in the Dueber YTK system
65194pYTK087Encodes LEU2 3' Homology as a Type 7 part to be used in the Dueber YTK system
65195pYTK088Encodes HO 3' Homology as a Type 7 part to be used in the Dueber YTK system
65196pYTK089Encodes AmpR-ColE1 as a Type 8a part to be used in the Dueber YTK system
65197pYTK090Encodes KanR-ColE1 as a Type 8a part to be used in the Dueber YTK system
65198pYTK091Encodes SpecR-ColE1 as a Type 8a part to be used in the Dueber YTK system
65199pYTK092Encodes URA3 5' Homology as a Type 8b part to be used in the Dueber YTK system
65200pYTK093Encodes LEU2 5' Homology as a Type 8b part to be used in the Dueber YTK system
65201pYTK094Encodes HO 5' Homology as a Type 8b part to be used in the Dueber YTK system
65202pYTK095Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK system
65203pYTK096Pre-Assembled Ura3 integration vector to be used in the Dueber YTK system.

Antibodies from Article