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Recombination-induced tag exchange to track old and new proteins.

Verzijlbergen KF, Menendez-Benito V, van Welsem T, van Deventer SJ, Lindstrom DL, Ovaa H, Neefjes J, Gottschling DE, van Leeuwen F
Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):64-8. doi: 10.1073/pnas.0911164107. Epub 2009 Dec 14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
64752pFvL118Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection
64755pTW081Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection
64766pKV015Recombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a T7-RFP tag, invariable V5 tag, Hygro selection

Antibodies from Article