pKV015
(Plasmid
#64766)
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PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a T7-RFP tag, invariable V5 tag, Hygro selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64766 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeRITE cassette
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRITE cassette
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SpeciesSynthetic
- Promoter none
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKV015 was a gift from Fred van Leeuwen (Addgene plasmid # 64766 ; http://n2t.net/addgene:64766 ; RRID:Addgene_64766) -
For your References section:
Recombination-induced tag exchange to track old and new proteins. Verzijlbergen KF, Menendez-Benito V, van Welsem T, van Deventer SJ, Lindstrom DL, Ovaa H, Neefjes J, Gottschling DE, van Leeuwen F. Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):64-8. doi: 10.1073/pnas.0911164107. Epub 2009 Dec 14. 10.1073/pnas.0911164107 PubMed 20018668