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Addgene

px330 Rosa26 AscI LH291Gu1 (LH500)
(Plasmid #99628)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99628 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    px330_U6_Chimeric
  • Total vector size (bp) 8509
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    px330 Rosa26 AscI LH291Gu1 (LH500) was a gift from Rui Benedito (Addgene plasmid # 99628 ; http://n2t.net/addgene:99628 ; RRID:Addgene_99628)

  • For your References section:

    Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis. Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R. Cell. 2017 Aug 10;170(4):800-814.e18. doi: 10.1016/j.cell.2017.07.031. 10.1016/j.cell.2017.07.031 PubMed 28802047
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