YIp204-PADH1-atTIR1-9myc
(Plasmid #99532)

• Purpose: S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
• Depositing Lab: Helle Ulrich
• Publication: Unpublished Ulrich lab AID plasmids (unpublished)

Backbone

• Vector backbone: YIplac204
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TIR1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2909
• Entrez Gene: TIR1 (a.k.a. AT3G62980, AtTIR1, TRANSPORT INHIBITOR RESPONSE 1)
• Promoter: ADH1
• Tag / Fusion Protein:
  • 9xMyc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XmaI (unknown if destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The TIR1 gene was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Digest this vector with EcoRV for integration into TRP1.

Addgene NGS results found a single nucleotide change compared to NM_116163.4 that results in T591I within the TIR1 translation. The protein is noted by the depositing laboratory to be functional in vivo