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Addgene

YIp204-PADH1-AFB2
(Plasmid #99531)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99531 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YIplac204
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AFB2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2508
  • Entrez Gene
    AFB2 (a.k.a. AT3G26810, auxin signaling F-box 2)
  • Promoter ADH1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site PstI (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The AFB2 gene was a kind gift from Jennifer L. Nemhauser (University of Washington, Seattle, USA).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Digest vector with EcoRV for integration into TRP1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YIp204-PADH1-AFB2 was a gift from Helle Ulrich (Addgene plasmid # 99531 ; http://n2t.net/addgene:99531 ; RRID:Addgene_99531)
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