pHyg-AID*-6HA
(Plasmid #99520)

• Purpose: C-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae
• Depositing Lab: Helle Ulrich
• Publication: Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.1002/yea.2967. Epub 2013 Jul 23.

Backbone

• Vector backbone: pFA6a derivative
• Vector type: PCR template for yeast tagging cassette
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IAA17(71-116)
• Alt name: AID (Auxin-inducible degron)
• Alt name: AXR3
• Species: A. thaliana (mustard weed)
• Entrez Gene: AXR3 (a.k.a. AT1G04250, AUXIN RESISTANT 3, AtIAA17, F19P19.31, F19P19_31, IAA17, indole-3-acetic acid inducible 17)
• Tag / Fusion Protein:
  • 6xHA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: CGTACGCTGCAGGTCGAC
• 3′ sequencing primer: TCGATGAATTCGAGCTCG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The parent vector encoding AID was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry