pDB023k
(Plasmid #99219)

• Purpose: Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
• Depositing Lab: David Liu
• Publication: Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474.

Backbone

• Vector backbone: ColE1
• Backbone manufacturer: David Liu Lab
• Backbone size w/o insert (bp): 2729
• Total vector size (bp): 5624

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ppsp [SD8] T7RNAP(S12*,S203*,S527*)
• Species: Synthetic
• Insert Size (bp): 2895
• Mutation: amber codons at positions 12, 203, and 527 of T7 RNA polymerase
• Promoter: Ppsp

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pDB023k.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry