pDB007ns2a
(Plasmid
#99214)
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PurposeAccessory plasmid for PACE of PylRS variants; negative selection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99214 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneSC101
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Backbone manufacturerDavid Liu Lab
- Backbone size w/o insert (bp) 2921
- Total vector size (bp) 8302
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePpsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
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SpeciesSynthetic
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Insert Size (bp)5381
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Mutationamber codons at positions 12 and 203 of T7 RNA polymerase
- Promoter Ppsp, PproK, and Ptet
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDB007ns2a was a gift from David Liu (Addgene plasmid # 99214 ; http://n2t.net/addgene:99214 ; RRID:Addgene_99214) -
For your References section:
Continuous directed evolution of aminoacyl-tRNA synthetases. Bryson DI, Fan C, Guo LT, Miller C, Soll D, Liu DR. Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474. 10.1038/nchembio.2474 PubMed 29035361
