pDB007ns2a
(Plasmid #99214)

• Purpose: Accessory plasmid for PACE of PylRS variants; negative selection
• Depositing Lab: David Liu
• Publication: Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474.

Backbone

• Vector backbone: SC101
• Backbone manufacturer: David Liu Lab
• Backbone size w/o insert (bp): 2921
• Total vector size (bp): 8302

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
• Species: Synthetic
• Insert Size (bp): 5381
• Mutation: amber codons at positions 12 and 203 of T7 RNA polymerase
• Promoter: Ppsp, PproK, and Ptet

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pDB007ns2a.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry