pAAV-GFAP-mKate2.5f
(Plasmid #99129)

• Purpose: Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoter
• Depositing Lab: Viviana Gradinaru
• Publication: Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179. doi: 10.1038/nn.4593. Epub 2017 Jun 26.

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mKate2.5f
• Insert Size (bp): 3283
• Promoter: GFAP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AflII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)

Resource Information

• Supplemental Documents:
  • pAAV-GFAP-mKate2.5f.gb
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositing laboratory has confirmed that sequence discrepancies between the Addgene NGS Result and the provided pAAV-GFAP-mKate2.5f.gb do not affect plasmid function