pAAV-CAG-mRuby2
(Plasmid #99123)

• Purpose: An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter
• Depositing Lab: Viviana Gradinaru
• Publication: Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179. doi: 10.1038/nn.4593. Epub 2017 Jun 26.

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mRuby2
• Insert Size (bp): 3299
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRI/HindIII (not destroyed)

Resource Information

• Supplemental Documents:
  • pAAV-CAG-mRuby2.gb
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry