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Addgene

YFP Rab4
(Plasmid #99018)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6790
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab4
  • Species
    D. melanogaster (fly), Synthetic
  • Insert Size (bp)
    789
  • GenBank ID
    NM_166240.3
  • Entrez Gene
    Rab4 (a.k.a. Dmel_CG4921, CG 4921, CG4921, DRAB4, DmRab4, Dmel\CG4921, Q7KY04, RAB4c, rab4)
  • Promoter CMV
  • Tag / Fusion Protein
    • YFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Matthew Scott Addgene #37690

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

YFP Rab4 from Addgene 37690 was transferred to pcDNA3.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP Rab4 was a gift from Narasimhan Gautam (Addgene plasmid # 99018 ; http://n2t.net/addgene:99018 ; RRID:Addgene_99018)

  • For your References section:

    Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. O'Neill PR, Castillo-Badillo JA, Meshik X, Kalyanaraman V, Melgarejo K, Gautam N. Dev Cell. 2018 Jun 15. pii: S1534-5807(18)30422-2. doi: 10.1016/j.devcel.2018.05.029. 10.1016/j.devcel.2018.05.029 PubMed 29937389
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