pcDNA3-CXCL12-sfGFP
(Plasmid #98961)

• Purpose: Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12
• Depositing Lab: Erik Procko
• Publication: Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5344
• Total vector size (bp): 6379
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CXCL12
• Alt name: SDF-1
• Alt name: SDF1
• Alt name: SDF1alpha
• Species: H. sapiens (human)
• Insert Size (bp): 1035
• Mutation: Full-length, wildtype
• GenBank ID: NM_199168.3
• Entrez Gene: CXCL12 (a.k.a. IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Superfolder GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry