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Addgene

MSP577
(Plasmid #98801)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98801 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone manufacturer
    ATCC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pET28 MBP-HNLYFQS-TEV I138T, N171D, N176T
  • Species
    Synthetic
  • Mutation
    I138T, N171D, N176T

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSP577 was a gift from David Liu (Addgene plasmid # 98801 ; http://n2t.net/addgene:98801 ; RRID:Addgene_98801)

  • For your References section:

    Phage-assisted continuous evolution of proteases with altered substrate specificity. Packer MS, Rees HA, Liu DR. Nat Commun. 2017 Oct 16;8(1):956. doi: 10.1038/s41467-017-01055-9. 10.1038/s41467-017-01055-9 [pii] PubMed 29038472
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