C321.ΔClpS.Ub-UAG-sfGFP
(Bacterial strain #98565)

• Purpose: Recoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter and ClpS inactivated
• Depositing Lab: George Church
• Publication: Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;115(3):619-624. doi: 10.1073/pnas.1715137115. Epub 2018 Jan 4.

Backbone

• Vector backbone: C321.deltaA genome (genomically integrated construct) with ClpS inactivated
• Backbone size w/o insert (bp): 4600000
• Modifications to backbone: A reporter construct was integrated into an intergenic region of the E. coli genome. Subsequently, the N-end rule adaptor protein ClpS was inactivated using MAGE (insertion of multiple premature stop codons).
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): C321.deltaA
• Growth instructions: The C321.deltaA strain contains an integrated lambda red cassette under expression by heat shock. Growth at 34 C or lower is recommended to prevent leaky expression of lambda red.
• Copy number: Unknown

Resource Information

• Supplemental Documents:
  • genomically-integrated-reporter.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry