miR-vector plix4.03
(Plasmid
#97141)
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Purpose(Empty Backbone) For lentiviral tet-inducible expression of miRNA and Dendra2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 97141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneplix 4.03
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Backbone manufacturerDavid Root (Addgene plasmid # 41395)
- Backbone size (bp) 7700
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Vector typeLentiviral
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Tag
/ Fusion Protein
- dendra2 (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- dendra2 (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
infusion cloning was used
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
miR-vector plix4.03 was a gift from Qing Deng (Addgene plasmid # 97141 ; http://n2t.net/addgene:97141 ; RRID:Addgene_97141) -
For your References section:
Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration. Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, Umulis DM, Wan J, Deng Q. Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 10.1073/pnas.1905221116. 10.1073/pnas.1905221116 PubMed 31451657
