pDHt/sk-CC
(Plasmid
#92127)
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PurposeExpression of codon-optimized Cas9 with Pcbh1 promoter in Trichoderma reesei
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 92127 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDHt/sk
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Vector typeCRISPR ; Trichoderma reesei expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9
- Promoter Pcbh1
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDHt/sk-CC was a gift from Zhihua Zhou (Addgene plasmid # 92127 ; http://n2t.net/addgene:92127 ; RRID:Addgene_92127) -
For your References section:
Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system. Liu R, Chen L, Jiang Y, Zhou Z, Zou G. Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. eCollection 2015. 10.1038/celldisc.2015.7 PubMed 27462408