YopH-pNIC-Bsa4
(Plasmid
#92063)
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PurposepNIC expression venctor encoding gene for YopH
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 92063 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepNIC28-Bsa4
- Backbone size w/o insert (bp) 5356
- Total vector size (bp) 6277
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYopH
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SpeciesYersinia pestis
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Insert Size (bp)921
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MutationCodon optimized E. coli
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Tag
/ Fusion Protein
- 6x His Tag (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TACTTCCAATCCATG
- 3′ sequencing primer TATCCACCTTTACTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YopH-pNIC-Bsa4 was a gift from Josh Beckham (Addgene plasmid # 92063 ; http://n2t.net/addgene:92063 ; RRID:Addgene_92063)
