EhPTP-pNIC-Bsa4
(Plasmid
#92064)
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PurposeExpression vector containing gene for EhPTP
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 92064 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNIC28-Bsa4
- Backbone size w/o insert (bp) 5356
- Total vector size (bp) 5830
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameProtein Tyrosine Phosphatase
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SpeciesEntamoeba histolytica
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Insert Size (bp)474
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Tag
/ Fusion Protein
- 6x His tag (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TACTTCCAATCCATG
- 3′ sequencing primer TATCCACCTTTACTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EhPTP-pNIC-Bsa4 was a gift from Josh Beckham (Addgene plasmid # 92064 ; http://n2t.net/addgene:92064 ; RRID:Addgene_92064)