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pCAGIG_V5-PPP6C
(Plasmid #92013)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92013 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGIG
  • Backbone manufacturer
    Connie Cepko Lab (Addgene #11159)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPP6C
  • Species
    H. sapiens (human)
  • Entrez Gene
    PPP6C (a.k.a. PP6, PP6C)
  • Tag / Fusion Protein
    • V5 (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The coding sequence for PPP6C was obtained from the Invitrogen Ultimate ORF LITE Library (Clone ID IOH7224).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The fluorescent protein in this plasmid is mEmerald, but with F64 (a residue seen in unmodified GFP.)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGIG_V5-PPP6C was a gift from Joshua Mendell (Addgene plasmid # 92013 ; http://n2t.net/addgene:92013 ; RRID:Addgene_92013)

  • For your References section:

    An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Golden RJ, Chen B, Li T, Braun J, Manjunath H, Chen X, Wu J, Schmid V, Chang TC, Kopp F, Ramirez-Martinez A, Tagliabracci VS, Chen ZJ, Xie Y, Mendell JT. Nature. 2017 Feb 9;542(7640):197-202. doi: 10.1038/nature21025. Epub 2017 Jan 23. 10.1038/nature21025 PubMed 28114302
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