pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2
(Plasmid #91873)

• Purpose: Photo-activatable/light sensitive Vav2
• Depositing Lab: Klaus Hahn
• Publication: Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444.

Backbone

• Vector backbone: pBABE
• Total vector size (bp): 7200
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: LOV2
• Species: Synthetic
• Insert Size (bp): 447
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: GAGATCAAGCAGAGGCTGAAG
• 3′ sequencing primer: AAGTTCTTTTGCCGCCTCATC

Gene/Insert 2

• Gene/Insert name: VAV2 DH/PH/ZF
• Alt name: VAV2
• Species: M. musculus (mouse)
• Insert Size (bp): 1200
• Entrez Gene: Vav2 (a.k.a. 2810040F13Rik, AI847175, Vav-2)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: GAGATCAAGCAGAGGCTGAAG
• 3′ sequencing primer: AAGTTCTTTTGCCGCCTCATC

Resource Information

• Supplemental Documents:
  • 1756.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry